Vitek 2 technology represents a smarter way to automate idast testing. Vitek 2 instrument user manual i 51073110en1 general warnings important. The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana henry kwadwo hackman1, george oseiadjei1. Place a small barcode label near the top of a 3ml vitek saline tube. The vitek 2 system nextgeneration platform provides greater automation while increasing safety and eliminating repetitive manual operations. Biomerieux vitek 2 gram negative susceptibility test cards. Performance of vitek2 compact and overnight microscan. Microbial identification using the biomerieux vitek 2 system david h. To inoculate the vitek 2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min.
Gherardi g, angeletti s, panitti m, pompilio a, di bonaventura g, crea f, et al. The quiz enables you to measure your level of proficiency on the routine use of the software. Vitek 2 yeast caspofungin is a quantitative test intended for use with the vitek 2 and vitek 2 compact systems as a laboratory aid in the determination of in vitro susceptibility to antifungal agents. Description of the biomerieux vitek 2 gram negative susceptibility test cards, astgn04, 20pk. Vitek 2 identification id cards let you easily and safely test and identify a wide range of clinically relevant organisms. We evaluated the ability of the vitek 2 system biomerieux, inc, hazelwood, mo. Application proper use of the instrument is the responsibility of trained. It is concluded that the new vitek 2 gp identification card provides reliable results for the. I started my career with algorithms for speeding up some of the most frequent operations performed by object oriented programs, namely, method dispatch and type tests. Quality control summary vitek2, auto and manual dilution a s an additional qc study was performed with the vitek2 compact, the secondary option, at three sites, with the results in the following tables. Use, maintenance and quality control procedures scope the purpose of this sop is to describe the procedures for the preparation and identification of test microorganisms test microbes and quality control organisms using the vitek 2 compact instrument. Clinical isolates of staphylococcus aureus n 100, coagulasenegative staphylococci. The efficiency of the vitek 2 compact system relies on the advanced colorimetry technology.
Used biomerieux vitek 2 compact microbiology for sale. Evaluation of the vitek 2 system for identification and antimicrobial. Package insert is located in the supervisors office. Realtime pcr detection of shigatoxin producing escherichia coli stec, serotype o157 and non o157 in fresh produce and food with nono157 isolation and identification revision. Customer training course book and instrument user manual are maintained. For some isolates, other conventional methods such as api biome. United states department of agriculture agricultural. From the above results we can conclude that vitek 2 compact is an automated system and gives more accurate results. Observa is an information management system that stores the test data generated by the vitek 2 or vitek 2 compact. I proposed a compact solution to the dispatching problem in.
The reliability of using vitek 2 compact system to detect extendedspectrum betalactamaseproducing isolates in escherichia coli and klebsiella pneumoniae in accra, ghana henry kwadwo hackman1, george osei. To inoculate the vitek2 cards directly from a positive blood culture bottle, a 5ml aliquot was centrifuged at 60 g for 10 min. Us environmental protection agency office of pesticide programs. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of capacity and automation. In diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. We evaluated the ability of the vitek 2 system biomerieux, inc. Mandell, douglas and bennetts principle and practice of infectious diseases. April 1996 dhcp laboratory vitek literal interface la5. Modifications by biomerieux can be made without prior notice biomerieux, the blue logo, advanced expert system, bioart, densichek, myla, vilink and vitek are used, pending andor registered trademarks belonging to biomerieux, or one of its subsid iaries, or one of its. A study was conducted to evaluate the new vitek 2 system biomerieux for identification and antibiotic susceptibility testing of grampositive cocci. Shop online for a wide selection of microbiologics vitek 2 gp comprehensive qc set microorganism sets for culture purposes and qc applications. Malditof ms versus vitek 2 anc card for identification of. It is a fully automated system that eliminates the inherent bias of manual mic determination.
Vitek manual table of contents mount sinai hospital. The course the routine use of vitek 2 compact has been designed for those who need to operate the instrument in a routine mode without having to perform expert tasks. Rapid reliable identification of these organisms is essential for accurate diagnosis and prompt effective treatment. Vitek 2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures. Vitek 2 compact identification cards for example, gp, anc, bcl, and gn. Vitek 2 yeast caspofungin is designed for antifungal susceptibility testing of candida species. The fundamentals of vitek 2 compact operations are presented through lessons, tutorials and exercises to enable you to take the most out of the instrument. Office of pesticide programs microbiology laboratory. Performance of the vitek 2 system software version 5. Manufacturer alerts and bulletins are located the supervisors office. Prep station entering target slide and vitek 2 ast test cards duration.
Use of the vitek 2 system to identify and test the antifungal. This biomerieux vitek 2 compact with dc7100usdt hp compaq computer is. The aim of this study was to compare the application of phenotypic and molecular tools in bacterial identification on the example of gramnegative. Several manual and commercial identification methods have been developed and are. View and download vitek virtuoso series operation manual online. Vitek 2 xl vitek 2 compact vitek 2 0118 93192002gbe document andor pictures not legally binding.
Principles the vitek 2 is an automated microbiology system utilizing growthbased technology. Laboratorybased evaluation of the colorimetric vitek2 compact. Principles the vitek 2 is an automated microbiology system utilizing growth based technology. The innovative vitek 2 microbial identification system includes an expanded identification database, the most automated platform available, rapid results, improved confidence, with minimal training time. Place assembled card and transfer tube on filling stand with the long portion of the transfer tube into the test tube. Use of the vitek 2 system for rapid identification of. When compared to sequencing and biochemical testing, the vitek ms correctly identified the scientific group or family 93. Generating an epub file may take a long time, please be patient. Ergonomic analysis comparison of the vitek 2 and vitek 2 compact. Vitek2 compact system direct testing procedure idgn and astn 020 cards were used, respectively, for identification and susceptibility testing directly from positive bactalert fa and fn blood cultures.
Pdf vitek 2 automated identification system and kocuria. Vitek 2 compact knowledge assessment quiz instruments. Vitek virtuoso series operation manual pdf download. Malditof ms versus vitek 2 anc card for identification of anaerobic bacteria background. Id and ast approach from positive blood culture bottles using vitek 2 compact. Headtohead comparison of microflex lt and vitek ms systems. Application of routine diagnostic procedure, vitek 2 compact. The system reads the latest generation vitek test cards containing 64 wells to ensure accuracy every 15 minutes using three different wavelengths. The user is advised to read and understand all instructions in this manual to be able to derive the best performance from the vitek 2 instrument and the smart carrier station. Principles of the vitek 2 is an automated microbiology system utilizing growthbased technology. With this technique, more data is analyzed, which increases results and accuracy. The reliability of using vitek 2 compact system to detect. Headtohead comparison of microflex lt and vitek ms.
Inoculate organism into 3ml vitek saline tube, vortex and invert the tube 2 to 3 times to uniformly suspend inoculum. The system is available in three formats vitek 2 compact, vitek 2, and vitek 2 xl that differ in increasing levels of. Fast, accurate, resistanceoriented susceptibility test results. Vitek 2 uses unique id and ast cards the size and shape of a playing card. Features of the biomerieux vitek 2 gram negative susceptibility test cards. Performance of vitek2 compact and overnight microscan panels. Comparative evaluation of the vitek2 compact and phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of gramnegative and grampositive isolates. Quality control summary vitek2 compact, manual dilution inoculum density control was monitored using the densichek2 instrument. Vitek 2 automated identification system and kocuria kristinae. Hazelwood, mo, usa objective this chapter describes the vitek 2 automated microbiology system and its application in the identification of microorganisms. Subculture frozen or freeze dried isolates twice before setting up any vitek 2 cards.
The configuration that you have purchased is adapted to the legislation. Matrixassisted laser desorption ionization timeofflight mass spectrometry malditof ms is an accurate, rapid and inexpensive technique that has initiated a revolution in the clinical microbiology laboratory for identification of pathogens. Advances in life science and technology issn 22247181 paper issn 2225062x online vol. The information is then gathered and formatted into many vital reports, including epidemiology and statistical reports. Jan 25, 2016 in diagnostic microbiology as well as in microbiological research, the identification of a microorganism is a crucial and decisive stage. Us environmental protection agency office of pesticide. The vitek 2 compact system biomerieux,france was used for confirmation of identified bacteria 12. Comparative evaluation of the vitek 2 compact and phoenix systems for rapid identification and antibiotic susceptibility testing directly from blood cultures of gramnegative and grampositive isolates. Microbial identification using vitek 2 pdf pdadhi parenteral. Virtuoso series security camera pdf manual download. The quiz the routine use of vitek 2 compact knowledge assessment quiz has been designed for those who need to use the vitek 2 compact in a daily routine. Scan cassette worksheet at the work station vitek 2 is designed to make your idast workflow as rapid and reliable as possible, while providing maximum flexibility and full traceability.
Staphylococci are an increasing cause of bloodstream infections. Ensure highperformance products, reproducible clinical test results and consistent microbiological evaluation of pharmaceuticals and food with thermo scientific cultiloops quality control organisms. The vitek 2 compact system is an automated microbiology bacterial identification. Vitek 2 susceptibility results obtained with the astp507 card and analysed with the advanced expert system software v.
Use of the vitek 2 system for rapid identification of clinical. Place filling stand with the assembled test kit into filling module. The vitek 2 advanced expert system software is like having an expert advisor standing by your side. Choose isolate prepare organism suspension and ensure correct mcfarland standard with densichek plus vitek 2 compact. Nov 30, 20 the reliability of using vitek 2 compact system to detect 1. Package insert contains system performance data and describes testing principle and procedure, qc recommendations, and limitations. The vitek 2 advanced expert system software is like having an. Used biomerieux vitek2 compact microbiology analyzer for. Thermo scientific vitek 2 qc sets include a readytouse set of qc organisms in cultiloops for. The reliability of using vitek 2 compact system to detect 1. Evaluation of the vitek 2 system for identification and.
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